UKKi008-A
NP0016-3
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UKKi008-A |
Alternative name(s) |
NP0016-3
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Notes | Please note that publication Pubmed ID: 24349418 only describes relevant patient characteristics, UKKi008-A is not included in the iPSC lines published. |
Provider |
|
Depositor | Klinikum der Universität zu Köln (UKK) |
Owner | Institute for Neurophysiology, Medical Faculty |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
|
External Databases |
|
hPSCreg | UKKi008-A |
BioSamples | SAMEA4583697 |
Cellosaurus | CVCL_9S60 |
ECACC | 66540015 |
CLO | CLO_0101550 |
Wikidata | Q54990425 |
General Information |
|
Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Age of donor (at collection) | 30-34 |
Ethnicity | Caucasian, German |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
|
Disease associated phenotypes |
|
Family history | Not known |
Is the medical history available upon request? | Yes |
Is clinical information available? | Medication information available |
External Databases (Donor) |
|
BioSamples | SAMEA4583720 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 30-34 |
Collected in | 2009 |
Passage number reprogrammed | P3 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Transposon (Sleeping beauty) |
Genes | |
Is the used vector excisable? |
Yes |
Absence of reprogramming vector(s)? |
Yes |
Reprogramming vectors silenced? |
Yes |
Methods used |
PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
miRNA
|
Other |
|
Selection criteria for clones | human ESC morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | Essential E8 |
Passage method | EDTA |
Surface coating | Vitronectin |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-80 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
SOX17 |
No |
FOXA2 |
No |
GSC |
Yes |
GATA6 |
No |
CXCR4 |
Yes |
PITX1 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
HAND1 |
Yes |
VIMENTIN |
Yes |
BMP4 |
Yes |
GATA4 |
Yes |
DCN |
Yes |
PECAM1 (CD31) |
Yes |
PDGF |
No |
In vitro spontaneous differentiation
Marker | Expressed |
PAX6 |
Yes |
Sox1 |
Yes |
B-TUBULIN |
No |
NEUROD1 |
Yes |
HES5 |
Yes |
FOXG1 |
Yes |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Not done |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
Normal male chromosome complement and banding pattern.
Passage number: P21
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Molecular karyotyping using OmniExpress Exome Chip
|