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UKKi008-A

NP0016-3

iPSC line

Re-stocking
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name UKKi008-A
Alternative name(s)
NP0016-3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Notes Please note that publication Pubmed ID: 24349418 only describes relevant patient characteristics, UKKi008-A is not included in the iPSC lines published.

Provider
Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine

External Databases
hPSCreg UKKi008-A
BioSamples SAMEA4583697
Cellosaurus CVCL_9S60
ECACC 66540015
CLO CLO_0101550
Wikidata Q54990425

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information
Sex male
Age of donor (at collection) 30-34
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Prolonged QT interval
Prolonged QT interval in the OLS
Heterozygous mutation in SCN5A gene c.718G>A, p.V240M in the cytoplasmic loop between membrane-spanning segments four and five within the first domain (DI-S4/S5) of Nav1.5 channel
The donor is affected.
Stage
Symptomatic
Synonyms
  • Long QT syndrome
  • Prolong qt interval on ekg
Genetic variants
SCN5A (target)
3p22.2
NM_000335.4:c.718G>A
NP_000326.2:p.V240M
Heterozygous
24349418
See publication Pubmed ID: 24349418 for relevant patient characteristics.
Disease associated phenotypes
  • Long QT syndrome type 3
Family history Not known
Is the medical history available upon request? Yes
Is clinical information available? Medication information available

External Databases (Donor)
BioSamples SAMEA4583720

hIPSC Derivation#

General
Source cell type
fibroblast of dermis
fibroblast of dermis in the OLS
Source cell origin
zone of skin
zone of skin in the OLS
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 30-34
Collected in 2009
Passage number reprogrammed P3

Reprogramming method
Vector type Integrating
Vector Transposon (Sleeping beauty)
Genes
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Expression_of_transposon-encoded_repr-factors_NP0016-3-iPSC.jpg

Vector free reprogramming
Type of used vector free reprogramming factor(s)
miRNA

Other
Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Brightfield image of NP0016-3 iPSC colonies at day 1 post-thaw; 10x objective
Brightfield image of NP0016-3 iPSC colonies at day 1 post-thaw at higher magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
No
FOXA2
No
GSC
Yes
GATA6
No
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
Yes
DCN
Yes
PECAM1 (CD31)
Yes
PDGF
No
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
B-TUBULIN
No
NEUROD1
Yes
HES5
Yes
FOXG1
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Normal male chromosome complement and banding pattern.
UKKi008-A.PNG
Passage number: P21
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes
Molecular karyotyping using OmniExpress Exome Chip