UKKi016-B

NP0078-12

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi016-B
Alternative name(s)
NP0078-12
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi016-A
(NP0078-10)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-C
(NP0078-13)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-A
(NP0077-6D)
Donor's gene variants:
KCNQ1
Donor diseases:
Prolonged QT interval
UKKi029-B
(NP0077-16E)
Donor's gene variants:
KCNQ1
Donor diseases:
Prolonged QT interval
UKKi029-C
(NP0077-18C)
Donor's gene variants:
KCNQ1
Donor diseases:
Prolonged QT interval
UKKi034-A
(NP0079-A, NP0079-7B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
UKKi034-B
(NP0079-B, NP0079-15B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
UKKi034-C
(NP0079-C, NP0079-16H)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
long QT syndrome 1
TAUi006-A
(UTA.00102.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi006-B
(UTA.00118.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi007-A
(UTA.00208.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
TAUi007-B
(UTA.00211.LQT1)
Donor's gene variants:
KCNQ1 – potassium voltage-gated channel subfamily Q member 1
Donor diseases:
Long QT Syndrome 1
LUMCi039-A
(LQT1-1781G/A hiPSC, LUMC0021iKCNQ-30)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Long QT Syndrome 1
UKKi023-A
(NP0126-1)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-B
(NP0126-5)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-C
(NP0126-6)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi035-B
(NP0139-B, NP0139-6C)
Donor's gene variants:
MYBPC3, MYBPC3
Donor diseases:
Hypertrophic cardiomyopathy
UKKi018-C
(NP0080-8B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-A
(NP0080-2B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-A
(NP0081-1A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-B
(NP0080-6A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-B
(NP0081-11)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-C
(NP0081-12C)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi017-A
(NP0075-8D)
Donor diseases:
Hypertrophic cardiomyopathy
UKKi025-A
(NP0135-1)
Donor's gene variants:
MYH7
Donor diseases:
Hypertrophic cardiomyopathy
UKKi017-B
(NP0075-10K)
Donor diseases:
Hypertrophic cardiomyopathy
UKKi017-C
(NP0075-11B)
Donor diseases:
Hypertrophic cardiomyopathy
UKKi025-B
(NP0135-2)
Donor's gene variants:
MYH7
Donor diseases:
Hypertrophic cardiomyopathy
UKKi025-C
(NP0135-7)
Donor's gene variants:
MYH7
Donor diseases:
Hypertrophic cardiomyopathy
UKKi024-A
(NP0133-5)
Donor diseases:
Brugada syndrome
UKKi024-B
(NP0133-1)
Donor diseases:
Brugada syndrome
UKKi024-C
(NP0133-4)
Donor diseases:
Brugada syndrome
ITXi003-A
(MS368)
Donor diseases:
Long QT Syndrome
ISMMSi044-A
(PLNR14del-1BC1)
Donor diseases:
Cardiomyopathy
ISMMSi045-A
(PLNR14del-2BC4)
Donor diseases:
Cardiomyopathy
ISMMSi047-A
(PLN-R14del-4AC4)
Donor diseases:
Cardiomyopathy
ISMMSi048-A
(PLN-R14del-5BC4)
Donor diseases:
Cardiomyopathy
SCVIi037-A
(SCVI672)
Donor diseases:
hypertrophic cardiomyopathy
FAMRCi009-A
(RCMP43)
Donor's gene variants:
FLNC
Donor diseases:
Restrictive Cardiomyopathy
ICGi028-A
(HCM4fm5.2)
Donor diseases:
Hypertrophic Cardiomyopathy
SCVIi050-A
(SCVI2486)
Donor diseases:
Dilated Cardiomyopathy
BRCi021-A
(TUH1)
Donor diseases:
Cardiomyopathy

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases

hPSCreg UKKi016-B
BioSamples SAMEA104615855
ECACC 66540887
Cellosaurus CVCL_VF34
CLO CLO_0101639
Wikidata Q54990432

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 30-34
Ethnicity caucasian / european

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Familial long QT syndrome
Long QT syndrome 1, c. 1096 C>T, p. Arg366Trp
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Congenital long QT syndrome
  • LQTS
Genetic variants
KCNQ1 (target)
11p15.5-p15.4
NM_000218.2:c.1096 C>T
NP_000209.2: p. Arg366Trp
Heterozygous
Please explain briefly the supporting evidence
Family history brother + son + niece + grandniece also affected
Is the medical history available upon request? yes
Is clinical information available? yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104615890

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 30-34
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT4
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations to be reported.
Passage number: 17
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes