UKKi034-C
NP0079-C, NP0079-16H
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UKKi034-C |
Alternative name(s) |
NP0079-C, NP0079-16H
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
Depositor | Klinikum der Universität zu Köln (UKK) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
Derivation country | Germany |
External Databases |
|
hPSCreg | UKKi034-C |
BioSamples | SAMEA104615858 |
ECACC | 66540894 |
Cellosaurus | CVCL_VF41 |
CLO | CLO_0102287 |
Wikidata | Q54990540 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 30-34 |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
Family history | no |
Is the medical history available upon request? | yes |
Is clinical information available? | yes |
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
Yes
|
Donor Relations |
|
Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA104615902 |
hIPSC Derivation#
General |
|
Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
|
Age of donor (at collection) | 30-34 |
Collected in | 2017 |
Passage number reprogrammed | 0 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Selection criteria for clones | morphology |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: StemFlex
Main protein source: Serum concentration: % |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
No larger chromosomal aberrations to be reported; Chr15: Duplication in q11.2 on
Passage number: 20
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
|