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UKKi018-A

NP0080-2B

iPSC line

Re-stocking
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluency
Magnification: 4x
Timepoint: Confluency
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name UKKi018-A
Alternative name(s)
NP0080-2B
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider
Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases
hPSCreg UKKi018-A
BioSamples SAMEA17622418
Cellosaurus CVCL_LD25
ECACC 66540478
CLO CLO_0101618
Wikidata Q54990438

General Information
Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information
Sex female
Age of donor (at collection) 20-24
Ethnicity caucasian / european

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Familial long QT syndrome
Familial long QT syndrome in the OLS
Disease variant: familial long QT syndrome 2, KCNH2, c.173 A>G; p.Glu58Gly
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Congenital long QT syndrome
  • LQTS
Genetic variants
KCNH2 (target)
7q36.1
NM_000238.3:c.173 A>G
NP_000229.1:p.Glu58Gly
Heterozygous
Please explain briefly the supporting evidence
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA17623168

hIPSC Derivation#

General
Source cell type
peripheral blood mononuclear cell
peripheral blood mononuclear cell in the OLS
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 20-24
Collected in 2016
Source cell line vendor University Hospital Münster, Germany
Passage number reprogrammed 0

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
Immunostaining, PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced
sendai_absence_NP0080-2B.png

Vector free reprogramming
Type of used vector free reprogramming factor(s)
None

Other
Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium mTeSR1
Passage method EDTA
Surface coating Matrigel
O2 concentration 21
CO2 concentration 5
Temperature 37 °C
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
Essential 8 supplement 50x 10 ml
Essential 8 Flex supplement 50x 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b
Yes
–
FOXD3
Yes
–
NANOG
Yes
–
–
OCT 4
Yes
–
–
SOX2
Yes
–
SSEA-1
Yes
–
SSEA-4
Yes
–
–
TRA 1-80
Yes
–
–
CD90
Yes
–
SSEA-1
No
–
POU5F1 (OCT-4)
Yes
–
SSEA-4
Yes
–
TRA 1-60
Yes
–
Morphology pictures
morphology pictures of iPS cell line NP0080-2B before banking and of the thawing control
morphology pictures of iPS cell line NP0080-2B before banking and of the thawing control
morphology pictures of iPS cell line NP0080-2B before banking and of the thawing control
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
SOX17
Yes
CXCR4
Yes
Gata6
Yes
Morphology
expression of SOX17 in differentiated iPS cell line NP0080-2B
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
VIM
Yes
DCN
Yes
MIXL1
Yes
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0080-2B
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
NEUROD1
Yes
PAX6
Yes
HES5
Yes
Morphology
expression of NESTIN in differentiated iPS cell line NP0080-2B

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations observed
Karyology_NP0080-2B.png
Passage number: 33
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)