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UKKi009-B

NP0011-19

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
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General#

Cell Line

hPSCreg name UKKi009-B
Alternative name(s)
NP0011-19
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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KCNH2
Donor diseases:
Familial long QT syndrome
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KCNQ1, KCNQ1
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(NP0077-16E)
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KCNQ1
Donor diseases:
Familial long QT syndrome
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(NP0077-18C)
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UKKi034-A
(NP0079-A, NP0079-7B)
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KCNQ1, KCNQ1
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Familial long QT syndrome
UKKi034-B
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KCNQ1, KCNQ1
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Familial long QT syndrome
STBCi096-A
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Familial long QT syndrome
UKKi016-B
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KCNQ1
Donor diseases:
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UKKi016-C
(NP0078-13)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
Derivation country Germany

External Databases

hPSCreg UKKi009-B
BioSamples SAMEA2825897
Cellosaurus CVCL_9S62
Wikidata Q54990427

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 35-39
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Long QT (LQT) Syndrome Type 2, mutation in KCNH2 gene c.3035_3045delTCCCTCGATGC, p.Leu1012Profs*55
The donor is affected.
Stage
Symptomatic
Synonyms
  • Congenital long QT syndrome
  • LQTS
Genetic variants
KCNH2 (target)
7q36.1
1.NM_000238.3: c.3035_3045delTCCCTCGATGC
NP_000229.1:p.Leu1012Profs*55
Please explain briefly the supporting evidence
Family history Not known
Is the medical history available upon request? Yes
Is clinical information available? Medication available

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
HumanOmniExpressExome-8 BeadChip v1.2 (Illumina, Inc.)

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4584076

hIPSC Derivation#

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 35-39
Collected in 2009
Passage number reprogrammed P3

Reprogramming method

Vector type Integrating
Vector Transposon (Sleeping beauty)
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Brightfield image of NP0011-19 iPSC colonies on day 2 post-thaw at high magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
No
GSC
Yes
GATA6
Yes
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
BMP4
Yes
GATA4
Yes
DCN
Yes
PECAM1 (CD31)
Yes
PDGF
No
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
B-TUBULIN
No
NEUROD1
Yes
HES5
Yes
FOXG1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 31
Karyotyping method: SNP-genotyping using HumanOmniExpressExome-8 BeadChip v1.2 (Illumina)

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes