We are currently making some changes to how orders are placed and because of this there will be a
short disruption period affecting order processing. Thank you for your patience while we work to
operate as smoothly as possible (November 14, 2023).
UKKi009-A
NP0011-8
iPSC line
Purchases have been temporarily disabled, as we are transitioning sales and distribution.
If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
If you are interested in purchasing this cell line, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
The EBiSC Access and Use Agreement must be completed along with an individual
Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org
for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | UKKi009-A |
| Alternative name(s) |
NP0011-8
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UKKi007-A (NP0014-6, UKK007Ai) Donor's gene variants: RYR2, RYR2 Donor diseases: Catecholaminergic polymorphic ventricular tachycardia UKKi007-B (NP0014-5) Donor's gene variants: RYR2 Donor diseases: Catecholaminergic polymorphic ventricular tachycardia |
Provider |
|
| Depositor | Klinikum der Universität zu Köln (UKK) |
| Owner | Institute for Neurophysiology, Medical Faculty |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
|
| Derivation country | Germany |
External Databases |
|
| hPSCreg | UKKi009-A |
| BioSamples | SAMEA4583816 |
| Cellosaurus | CVCL_9S61 |
| CLO | CLO_0101551 |
| Wikidata | Q54990426 |
General Information |
|
| Publications | View all related publications on hPSCreg (4) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 35-39 |
| Ethnicity | Caucasian, German |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
|
| Family history | Not known |
| Is the medical history available upon request? | Yes |
| Is clinical information available? | Medication available |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
HumanOmniExpressExome-8 BeadChip v1.2 (Illumina, Inc.)
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA4584076 |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblast of dermis |
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Age of donor (at collection) | 35-39 |
| Collected in | 2009 |
| Passage number reprogrammed | P3 |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Transposon (Sleeping beauty) |
| Genes | |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
Yes |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | human ESC morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | Essential E8 |
| Passage method | EDTA |
| Surface coating | Vitronectin |
| O2 concentration | 20 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
|
|||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| SSEA-4 |
Yes |
|
|
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| TRA 1-80 |
Yes |
|
|
Morphology pictures
Brightfield image of NP0011-8 iPSC colonies on day 2 post-thaw; 5x objective
Brightfield image of NP0011-8 iPSC colony on day 2 post-thaw at large magnification; 32x objective
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| FOXA2 |
No |
| GSC |
Yes |
| GATA6 |
Yes |
| CXCR4 |
Yes |
| PITX1 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| HAND1 |
Yes |
| VIMENTIN |
Yes |
| BMP4 |
Yes |
| GATA4 |
No |
| DCN |
Yes |
| PECAM1 (CD31) |
Yes |
| PDGF |
No |
In vitro spontaneous differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| Sox1 |
Yes |
| B-TUBULIN |
No |
| NEUROD1 |
Yes |
| HES5 |
Yes |
| FOXG1 |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Not done |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 30
Karyotyping method:
Molecular SNP karyotyping using OmniExpress Exome Chip
|
Other Genotyping (Cell Line) |
|