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UKKi016-A

NP0078-10

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
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General#

Cell Line

hPSCreg name UKKi016-A
Alternative name(s)
NP0078-10
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi016-B
(NP0078-12)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-C
(NP0078-13)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-C
(NP0079-C, NP0079-16H)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-A
(NP0077-6D)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-B
(NP0077-16E)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-C
(NP0077-18C)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-A
(NP0079-A, NP0079-7B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-B
(NP0079-B, NP0079-15B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi023-A
(NP0126-1)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-B
(NP0126-5)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-C
(NP0126-6)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-C
(NP0080-8B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-A
(NP0080-2B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-A
(NP0081-1A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-B
(NP0080-6A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-B
(NP0081-11)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-C
(NP0081-12C)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi008-A
(NP0016-3)
Donor's gene variants:
SCN5A
Donor diseases:
Familial long QT syndrome
STBCi096-A
(BPC340-03-06)
Donor diseases:
Familial long QT syndrome
UKKi009-B
(NP0011-19)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
Derivation country Germany

External Databases

hPSCreg UKKi016-A
BioSamples SAMEA104615854
Cellosaurus CVCL_VF33
Wikidata Q54990430

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 30-34
Ethnicity caucasian / european

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Long QT syndrome 1, c. 1096 C>T, p. Arg366Trp
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Congenital long QT syndrome
  • LQTS
Genetic variants
KCNQ1 (target)
11p15.5-p15.4
NM_000218.2:c.1096 C>T
NP_000209.2: p. Arg366Trp
Heterozygous
Please explain briefly the supporting evidence
Disease associated phenotypes
  • QTc: 501 ms
Family history brother + son + niece + grandniece also affected
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104615890

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 30-34
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT4
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations to be reported.
Passage number: 23
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes