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EBiSC-NEUR1

iPSC derived cells

Not-for-profit fee: £1127 per vial

Immediately available for distribution*

*Once all legal and processing details completed

Time: 14 days post-thaw
Magnification: 40x
Description: Cryopreserved EBiSC-NEUR1 neurons were thawed, cultured on Poly-Ornithine / Laminin and immunostained after 14 days for microtubule-associated protein 2 (MAP2, yellow), neuronal nuclei (NeuN, magenta) and DAPI (blue).

A Product Information Pack contains information about a product including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the derived product.

You will need to complete an EBiSC Access and Use Agreement (EAUA) before purchasing your cell line.

This Certificate of Analysis is of the latest released batch. We are publishing it for reference purpose, but we cannot guarantee batch specific delivery.

This user protocol is of the latest released batch. We are publishing it for reference purpose, but we cannot guarantee batch specific delivery.

EBiSC-NEUR1 neurons demonstrate robust and synchronous firing

EBiSC NEUR1 exhibit an excitatory glutamatergic phenotype

EBiSC NEUR1 express a defined set of cortical layer markers

Catalogue No.	EBiSC-NEUR1
Product Type	iPSC-derived neurons
iPSC Product Name	EBiSC-NEUR1
iPSC Product Cell Type	Neuron
Source iPSC Line Name	BIONi010-C-13
Donor Disease Status	Normal
Genetic Modification Description:	DOX inducible NEUROG2
Format	2ml cryovial
Cell Number	4x10^6
Morphology Description	EBiSC-NEUR1 neurons extend multiple processes after thaw and form neurite networks

Description:

EBiSC-NEUR1 cells are early neurons derived from human induced pluripotent stem cells using a doxycycline-inducible NGN2 expression system. The transcription-factor based differentiation approach accelerates neuron maturation while reducing cell culture variability. When thawed and cultured according to the instructions in the User protocol, the EBiSC-NEUR1 neurons express classical pan-neuronal markers such as beta-III-tubulin (TUBB3) and microtubule-associated protein 2 (MAP2) and form neurite networks. EBiSC-NEUR1 cultures exert a great homogeneity with low batch-to-batch variability providing an ideal starting point for diverse co-culture approaches as well as large-scale assays and high-throughput drug screenings.
Quality control is performed prior to release and includes sterility testing, morphological assessment, as well as flow cytometric and immunochemical analyses for the expression of neuronal markers.

Publications

Shih PY et al. Development of a fully human assay combining NGN2-inducible neurons co-cultured with iPSC-derived astrocytes amenable for electrophysiological studies. Stem cell research. 2021 Jul;54:102386.

Kwok CK et al. Scalable expansion of iPSC and their derivatives across multiple lineages. Reproductive toxicology (Elmsford, N.Y.). 2022 Sep;112:23-35.