If the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe please get in touch via Contact@EBiSC.org.

UKKi029-A

NP0077-6D

iPSC line

Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name UKKi029-A
Alternative name(s)
NP0077-6D
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi029-B
(NP0077-16E)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi029-C
(NP0077-18C)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-A
(NP0078-10)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-C
(NP0079-C, NP0079-16H)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-A
(NP0079-A, NP0079-7B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi034-B
(NP0079-B, NP0079-15B)
Donor's gene variants:
KCNQ1, KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-B
(NP0078-12)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi016-C
(NP0078-13)
Donor's gene variants:
KCNQ1
Donor diseases:
Familial long QT syndrome
UKKi018-C
(NP0080-8B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-A
(NP0080-2B)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-A
(NP0081-1A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi018-B
(NP0080-6A)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-B
(NP0081-11)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-C
(NP0081-12C)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-A
(NP0126-1)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-B
(NP0126-5)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-C
(NP0126-6)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi009-B
(NP0011-19)
Donor's gene variants:
KCNH2
Donor diseases:
Familial long QT syndrome
UKKi008-A
(NP0016-3)
Donor's gene variants:
SCN5A
Donor diseases:
Familial long QT syndrome
STBCi096-A
(BPC340-03-06)
Donor diseases:
Familial long QT syndrome

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
Derivation country Germany

External Databases

hPSCreg UKKi029-A
BioSamples SAMEA104494405
Cellosaurus CVCL_RM68
Wikidata Q54990505

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 20-24

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
LQT1 syndrome (a type of cardiac arrhythmia), KCNQ1; c.1690G>C; p.Asp564His
Synonyms
  • Congenital long QT syndrome
  • LQTS
Genetic variants
KCNQ1 (target)
11p15.5-p15.4
NM_000218.2:c.1690G>C
NP_000209.2:p.Asp564His
Heterozygous
Please explain briefly the supporting evidence
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104494406

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Source cell type (free text) Peripheral Blood Mononuclear Cells; IfGH Münster
Age of donor (at collection) 20-24
Collected in 2017
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
Immunostaining, PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: %
Supplements
Essential 8 50x 10 ml
StemFlex 10x 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT 4
Yes
SOX2
Yes
TRA 1-80
Yes
SSEA-4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations to be reported.
Passage number: 18
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)