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UKKi030-C

NP0134-26B

iPSC line

Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name UKKi030-C
Alternative name(s)
NP0134-26B
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi030-A
(NP0134-6D)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome
UKKi030-B
(NP0134-18A)
Donor's gene variants:
SCN5A
Donor diseases:
Brugada syndrome
UKKi008-A
(NP0016-3)
Donor's gene variants:
SCN5A
Donor diseases:
Familial long QT syndrome
UKKi024-A
(NP0133-5)
Donor diseases:
Brugada syndrome
UKKi024-B
(NP0133-1)
Donor diseases:
Brugada syndrome
UKKi024-C
(NP0133-4)
Donor diseases:
Brugada syndrome

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Distributors
EBiSC
Derivation country Germany

External Databases

hPSCreg UKKi030-C
BioSamples SAMEA104494412
Cellosaurus CVCL_RM73
Wikidata Q54990510

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
c.1936delC;p.Gln646Argfs*5, Exon 13, SCN5A-Gen
Synonyms
  • Idiopathic ventricular fibrillation, Brugada type
Genetic variants
SCN5A (target)
3p22.2
NM_000335.4:c.1936delC
NP_000326.2:p.Gln646Argfs*5
Heterozygous
Family history No
Is the medical history available upon request? No
Is clinical information available? Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104494410

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Source cell type (free text) UKK Cologne
Age of donor (at collection) 65-69
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR1
Passage method EDTA
Surface coating Matrigel
O2 concentration 21
CO2 concentration 5
Temperature 37 °C
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Albumine
Serum concentration: 0 %
Supplements
Stemflex 10x 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-80
Yes
OCT 4
Yes
NANOG
Yes
SSEA-4
Yes
SOX2
Yes
FOXD3
Yes
DNMT3b
Yes
SSEA-1
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
CXCR4
Yes
Gata6
Yes
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
VIM
Yes
DCN
No
MIXL1
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Unknown
HES5
Yes
NEUROD1
No
PAX6
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations to be reported.
Passage number: 18
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)