BIONi010-C-55
BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | BIONi010-C-55 |
Alternative name(s) |
BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Denmark |
External Databases |
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hPSCreg | BIONi010-C-55 |
BioSamples | SAMEA8837436 |
ECACC | 66770020 |
CLO | CLO_0103541 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ Plus
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46 XY, normal male karyotype
Passage number: 18
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification#
Genetic modifications not related to a disease |
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