BIONi010-C

BIONi010-C, K3P53

iPSC line

In Stock

At European Collection of Authenticated Cell Cultures (ECACC)

A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name	BIONi010-C
Alternative name(s)	BIONi010-C, K3P53
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	BIONi010-C-2 (BIONi010-C ApoE E3/E3 #H8 P32) BIONi010-C-3 (BIONi010-C ApoE KO #KO30 P30) BIONi010-C-15 (BIONi010-C +dox inducible NGN2-GFP) BIONi010-C-25 (BIONi010-C heterozygous TREM2 KO) BIONi010-C-6 (BIONi010-C ApoE E2/E2) BIONi010-C-7 (BIONi010-C Trem2 R47H) BIONi010-C-8 (BIONi010-C Trem2 T66M, #Y5-80) BIONi010-C-9 (BIONi010-C CD33 KO) BIONi010-C-17 (BIONi010-C TREM2 KO) BIONi010-C-24 (BIONi010-C Dox a-syn) BIONi010-C-4 (BIONi010-C ApoE E4/E4 #B44 P27) BIONi010-C-5 (BIONi010-C CD33 E2del #N14 P26) BIONi010-A (K1P53) BIONi010-B (K2P53, BIONi010-B) BIONi010-C-64 (BIONi010-C-T2A-Nanoluciferase reporter cl. 29) BIONi010-C-56 (BIONi010-C-A713T-C25) BIONi010-C-57 (BIONi010-C-A713T-C42) BIONi010-C-58 (BIONi010-C-A713T-C1) BIONi010-C-59 (BIONi010-C-A713T-C33) BIONi010-C-60 (BIONi010-C-R589C-C7) BIONi010-C-61 (BIONi010-C-R589C-C16) BIONi010-C-62 (BIONi010-C-R589C-C5) BIONi010-C-63 (BIONi010-C-R589C-C9) BIONi010-C-51 (BIONi010-C TNNI3-mCherry reporter) BIONi010-C-13 (BIONi010-C + NGN2 #I7-26) BIONi010-C-18 (BIONi010-C TBK1 KO) BIONi010-C-19 (BIONi010-C IKBKE KO) BIONi010-C-10 (HNF1AP291fsinsC +/- 54-5) BIONi010-C-11 (HNF1AP291fsinsC -/- 66-1) BIONi010-C-12 (HNF4ApR309C -/- 2-4) BIONi010-C-52 (BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6)) BIONi010-C-53 (BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2)) BIONi010-C-55 (BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74) BIONi010-C-43 (BIONi010-C + aSNCA-wt AAVS1) BIONi010-C-44 (BIONi010-C + aSNCA-A53T AAVS1) BIONi010-C-49 (BIONi010-C + synapsin-m2rtTA + SNCA-wt) BIONi010-C-50 (BIONi010-C + synapsin-m2rtTA + SNCA-A53T) BIONi010-C-54 (BIONi010-C with an APOE 4/4 genotype (with two functional alleles in contrast to BIONi010-C-4)) BIONi010-C-45 (BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6) BIONi010-C-41 (BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2) BIONi010-C-65 (BiONI010-C-O16) BIONi010-C-66 (BIONi010-C-N7) BIONi010-C-42 (BIONi010-C + iCRE AAVS1)
Notes	No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Provider
Depositor	Bioneer (BION)
Owner	Bioneer (BION)
Distributors	EBiSC European Collection of Authenticated Cell Cultures (ECACC)
Derivation country	Denmark
External Databases
hPSCreg	BIONi010-C
BioSamples	SAMEA3158050
Cellosaurus	CVCL_1E68
ECACC	66540023
CLO	CLO_0100558
Wikidata	Q54796750
General Information
Publications	Rasmussen MA et al. Transient p53 suppression increases reprogramming of human fibroblasts without affecting apoptosis and DNA damage. Stem cell reports. 2014 Sep 9;3(3):404-13. (reference publication) View all related publications on hPSCreg (66)
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclones	BIONi010-C-2 BIONi010-C-3 BIONi010-C-4 BIONi010-C-5 BIONi010-C-6 BIONi010-C-7 BIONi010-C-8 BIONi010-C-9 BIONi010-C-10 BIONi010-C-11 BIONi010-C-12 BIONi010-C-13 BIONi010-C-15 BIONi010-C-17 BIONi010-C-18 BIONi010-C-19 BIONi010-C-24 BIONi010-C-25 BIONi010-C-51 BIONi010-C-52 BIONi010-C-53 BIONi010-C-55

Donor Information#

General Donor Information

Sex

male

Age of donor (at collection)

15-19

Ethnicity

Black or African-American

Phenotype and Disease related information (Donor)

Diseases

No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples

SAMEA3105780

hIPSC Derivation#

General
Source cell line name	CC-2511 Derived from same source line (potentially other lot and donor, see below): BIONi010-A BIONi010-B
Source cell type	fibroblast of dermis fibroblast of dermis in the OLS
Source cell origin	zone of skin zone of skin in the OLS Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Source cell type (free text)	NHDF-Ad-Der Fibroblasts
Source cell line lot number	0000208364 Derived from same source line lot: BIONi010-A BIONi010-B
Age of donor (at collection)	15-19
Source cell line vendor	LONZA
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Genes	POU5F1 SOX2 KLF4 MYC Lin28 shP53
Is reprogramming vector detectable?	No
Methods used	RT-PCR
Vector free reprogramming
Other
Derived under xeno-free conditions	No
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzymatically Dispase
O2 Concentration	5 %
CO2 Concentration	5 %
Medium	mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
DNMT3B	Yes
SOX2	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
AFP	Yes

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Marker	Expressed
SMA	Yes

Ectoderm

Neuron
Ont Id: CL_0000540

In vitro spontaneous differentiation

In vitro directed differentiation

Marker	Expressed
Tuj1	Yes
NES	Yes
VIMENTIN	Yes
vGLUT1	Yes

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative
Mycoplasma	Negative

Sterility
Inoculation for microbiological growth	No Contaminants Detected
Mycoplasma	Not Detected
Viability	Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY O15-0117SLIDE1P45.jpg Passage number: 17 Karyotyping method: G-Banding
Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?	Yes SNP typing array No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 Whole genome sequencing https://ega-archive.org/studies/EGAS00001002755 This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768 Sanger Sequencing The genotype at base position described by rs429358 is T/C (ApoE3/E4) vcf File: BIONI010-C ApoE3-E4.PNG

BIONi010-C

BIONi010-C, K3P53

iPSC line

General#

Cell Line

Provider

External Databases

General Information

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Karyotyping (Donor)

Other Genotyping (Donor)

Donor Relations

External Databases (Donor)

hIPSC Derivation#

General

fibroblast of dermis

zone of skin

Reprogramming method

Vector free reprogramming

Other

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells

Differentiation Potency

Microbiology / Virus Screening

Sterility

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)