BIONi010-C-53
BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2)
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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| hPSCreg Name | BIONi010-C-53 |
| Alternative name(s) |
BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2)
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
Provider |
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| Depositor | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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| Derivation country | Denmark |
External Databases |
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| hPSCreg | BIONi010-C-53 |
| BioSamples | SAMEA8093246 |
| ECACC | 66770014 |
| CLO | CLO_0104077 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
|
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The source cell information can be found in the parental cell line
BIONi010-C.
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| Passage number reprogrammed | 36 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C .Genotyping#
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification#
| Genetic modifications not related to a disease |
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