BIONi010-C-45

BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6

Gene-edited iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name BIONi010-C-45
Alternative name(s)
BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider

Depositor Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg BIONi010-C-45
BioSamples SAMEA6232065
CLO CLO_0104216

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C .

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Gene knock-in
GBA1 gene, a LoxP site inserted in the intron before exon 5 and after exon 6
GBA1, A LoxP site inserted in the intron before exon 5 and after exon 6. After fluxing, by the induction of iCRE with doxycycline, a out-of-frame KO will be generated