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BIONi010-C-71

BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation

Gene-edited iPSC line

Not released.
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.

General#

Cell Line

hPSCreg name BIONi010-C-71
Alternative name(s)
BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation

Provider

Depositor Bioneer (BION)
Owner Bioneer (BSC)
Distributors
EBiSC
Derivation country Denmark

External Databases

hPSCreg BIONi010-C-71
BioSamples SAMEA115162593

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.
Passage number reprogrammed 32

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Medium mTeSR™ Plus

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-81
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 32

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
  • Alzheimer's disease
  • Alzheimer's disease, unspecified
show more synonyms
Genetic modifications
Variant
The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation