BIONi010-C-10
HNF1AP291fsinsC +/- 54-5
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | BIONi010-C-10 |
Alternative name(s) |
HNF1AP291fsinsC +/- 54-5
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Notes | No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 |
Provider |
|
Depositor | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
Derivation country | Denmark |
External Databases |
|
hPSCreg | BIONi010-C-10 |
BioSamples | SAMEA104619380 |
ECACC | 66541074 |
Cellosaurus | CVCL_RM84 |
CLO | CLO_0102724 |
Wikidata | Q54796752 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Subclone of |
Donor Information#
General Donor Information |
|
Sex | male |
Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
|
The source cell information can be found in the parental cell line
BIONi010-C.
|
|
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Episomal |
Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Method documentation
HNF1A 54-5 flow.tif
Flow cytometry of undifferentiated cells
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
|