BIONi010-C-18
BIONi010-C TBK1 KO
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | BIONi010-C-18 |
| Alternative name(s) |
BIONi010-C TBK1 KO
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Notes | No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 |
Provider |
|
| Depositor | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Denmark |
External Databases |
|
| hPSCreg | BIONi010-C-18 |
| BioSamples | SAMEA104491010 |
| ECACC | 66540637 |
| Cellosaurus | CVCL_RM89 |
| CLO | CLO_0102730 |
| Wikidata | Q54796763 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | male |
| Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
BIONi010-C.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Morphology 1 day after thawing
Method documentation
qPCR BIONi010-C-18.tif
qPCR analysis of undifferentiated cells
06-Sep-2017-Layout.tiff
Flow cytometry analysis of undifferentiated cells
Differentiation Potency
In vitro directed differentiation
Morphology
BIONi10-C18 endo d5 10x.tiff
Morphology of in vitro diff to endoderm
Protocol or reference
BIONi10-C18 endo d5.tiff
Flow Cytometry of in vitro differentiation to endoderm
In vitro directed differentiation
Morphology
BIONi10-C18 meso d5 10x.tiff
Morphology of in vitro diff to mesoderm
Protocol or reference
BIONi10-C18 meso d5.tiff
Flow Cytometry of in vitro differentiation to mesoderm
In vitro directed differentiation
Morphology
BIONi10-C18 ecto d5 10x.tiff
Morphology of in vitro diff to ectoderm
Protocol or reference
BIONi10-C18 ecto d5.tiff
Flow Cytometry of in vitro differentiation to ectoderm
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|