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DRICUi003-A

LC56A10005A

iPSC line

Not released.
The cell line is not released.

General#

Cell Line

hPSCreg name DRICUi003-A
Alternative name(s)
LC56A10005A
Cell line type Human induced pluripotent stem cell (hiPSC)
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Notes Further anonymized information about the donor including demographic and clinical data will be made available on the Dementia Platforms UK data portal: https://portal.dementiasplatform.uk/

Provider

Depositor UK Dementia Research Institute, Cardiff University (DRICU)
Owner UK Dementia Research Institute, Cardiff University (DRICU)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg DRICUi003-A
BioSamples SAMEA9362831
Cellosaurus CVCL_B3P1
Wikidata Q110432782

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 65-69
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Alzheimer Senile Dementia
  • late-onset Alzheimer's
  • Alzheimer Type Senile Dementia
  • Senile Dementia, Alzheimer Type
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA9362832

hIPSC Derivation#

General

Source cell type
A thymocyte-derived lymphocyte of immunological importance that is long-lived (months to years) and is responsible for cell-mediated immunity. T lymphocyte cells form rosettes with sheep erythrocytes and, in the presence of transforming agents (mitogens), differentiate and divide. These cells have the characteristic T3 surface marker and may be further divided into subsets according to function, such as helper, cytotoxic, etc.
Synonyms
  • T Cells
  • T Cell
  • T-Lymphocytes
  • Thymus-Dependent Lymphocytes
  • T cell
  • T-Lymphocyte
  • T-Cell
  • T lymphocyte
  • Thymus Derived Lymphocyte
  • T-Cells
show more synonyms
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Virus undetectable at Passage 9
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Advanced DMEM/F12 (Gibco Thermo Fisher 12634010)
Main protein source: Albumine
Serum concentration: 0 %
Supplements
GlutaMAX (Gibco Thermo Fisher 35050061) 1 %
Heparin (Stem Cell Technologies 07980) 0.1 µg/ml
Ascorbic Acid-2-phosphate magnesium salt (Sigma A8960) 0.22 mM
HEPES 1M pH 7.4 (Gibco Thermo Fisher 15630080) 15 mM
FGF-2 basic 145aa (Miltenyi Biotech 130-093-842) 100 ng/ml
TGF-beta (Biolegend 781804) 2 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
Morphology pictures
IPMAR_DRICUi003A_Morphology.pdf
Morphology of DRICUi003A on day 2 post-thaw
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
SRY-box transcription factor 17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
C-X-C Chemokine Receptor Type 4
Yes
BRACHYURY
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Paired box 6
Yes
orthodenticle homeobox 2
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No gross abnormalities
Passage number: 13
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)