If the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe please get in touch via Contact@EBiSC.org.

STBCi071-C

SFC057-07-02

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluency
Magnification: 4x
Timepoint: Confluency
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name STBCi071-C
Alternative name(s)
SFC057-07-02
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi071-A
(SFC057-07-03)
Donor diseases:
Alzheimer disease
STBCi071-B
(SFC057-07-01)
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-A
(SFC809-03-01)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi047-A
(SFC043-03-01)
Donor diseases:
Alzheimer disease
STBCi051-A
(SFC056-04-03)
Donor diseases:
Alzheimer disease
STBCi047-B
(SFC043-03-13)
Donor diseases:
Alzheimer disease
STBCi051-B
(SFC056-04-01)
Donor diseases:
Alzheimer disease
STBCi051-C
(SFC056-04-02)
Donor diseases:
Alzheimer disease
STBCi072-A
(SFC058-03-01)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-B
(SFC058-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-C
(SFC058-03-03)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi077-A
(SFC136-03-02)
Donor diseases:
Alzheimer disease
STBCi077-B
(SFC136-03-03)
Donor diseases:
Alzheimer disease
STBCi077-C
(SFC136-03-01)
Donor diseases:
Alzheimer disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
STBCi254-A
(SFC143-03-08)
Donor diseases:
Alzheimer disease
STBCi011-A
(SFC803-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-B
(SFC803-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-C
(SFC803-03-08)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-A
(SFC805-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-B
(SFC805-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-C
(SFC805-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-B
(SFC807-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-B
(SFC808-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi071-C
BioSamples SAMEA104493896
Cellosaurus CVCL_RC97
Wikidata Q54956574

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 75-79

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493812

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 75-79

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR1
Passage method EDTA
Surface coating Matrigel
O2 concentration 21
CO2 concentration 5
Temperature 37 °C
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
Gata6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
MIXL1
Yes
DCN
Yes
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NEUROD1
Yes
HES5
Yes
PAX6
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
minor Chromosome 7 lower copy number variation which is not present in parental fibroblasts
Passage number: 12
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)