STBCi099-A
SFC059-03-01
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | STBCi099-A |
Alternative name(s) |
SFC059-03-01
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
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Depositor | StemBANCC (STBC) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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hPSCreg | STBCi099-A |
BioSamples | SAMEA104615909 |
ECACC | 66540916 |
Cellosaurus | CVCL_RD68 |
CLO | CLO_0101943 |
Wikidata | Q54970239 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Donor Relations |
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Other cell lines of this donor |
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External Databases (Donor) |
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BioSamples | SAMEA104615910 |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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||||
TRA 1-60 |
Yes |
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Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
minor Chr6 allelic imbalance which is also present in parental fibroblasts
Passage number: 10
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |