STBCi015-C
SFC809-03-04
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | STBCi015-C |
Alternative name(s) |
SFC809-03-04
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UCLi004-B (RCFB60c7, RCi177) Donor's gene variants: C9orf72, C9orf72 Donor diseases: Amyotrophic lateral sclerosis Frontotemporal dementia UCLi004-C (RCi172, RCFB60c2) Donor's gene variants: C9orf72, C9orf72 Donor diseases: Amyotrophic lateral sclerosis Frontotemporal dementia LUBi001-B (PGRN-8310, RCFB58 c4.4, PGRN8310, RCi194) Donor's gene variants: GRN, GRN, GRN, GRN Donor diseases: Frontotemporal dementia LUBi001-C (PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310) Donor's gene variants: GRN, GRN, GRN, GRN Donor diseases: Frontotemporal dementia PFIZi013-A (RCi215, RCFB59 C9) Donor's gene variants: TARDBP, TARDBP Donor diseases: Amyotrophic lateral sclerosis |
Provider |
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Depositor | StemBANCC (STBC) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
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hPSCreg | STBCi015-C |
BioSamples | SAMEA104493624 |
ECACC | 66540666 |
Cellosaurus | CVCL_RB61 |
CLO | CLO_0101734 |
Wikidata | Q54956374 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
|
Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA104493622 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
|
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NANOG |
Yes |
|
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
Pass: minor allelic imbalance Chr3, 8,19; Chr3 and 19 also present in parent fibroblast. See CoA for additional abnormalities.
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |