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UKBi011-A-2

ApoE 2/2

Gene-edited iPSC line

Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
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General#

Cell Line

hPSCreg name UKBi011-A-2
Alternative name(s)
ApoE 2/2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A
(iLB-AD-169bm-s24)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A
(SFC140-04-01)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi072-A
(SFC058-03-01)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-B
(SFC058-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-C
(SFC058-03-03)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi256-A
(SFC144-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi037-A-2
(BIONi037-A ApoE2/2 #M10-7)
BIONi037-A-3
(BIONi037-A ApoE3/4 #P10-22)
BIONi037-A-4
(BIONi037-A ApoE4/4 #I10-53)
BIONi037-A-1
(16423 ApoE KO)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi061-A
(SFC850-03-01)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
STBCi062-A
(SFC851-03-04)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
DRICUi011-A
(BS38A10002A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi013-A
(SC45A10021A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi006-A
(ADANG10496CA)
Donor diseases:
late-onset Alzheimer's disease
DRICUi007-A
(ADANG10605CA)
Donor diseases:
late-onset Alzheimer's disease
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
DRICUi010-A
(BS38A10004A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi012-A
(NT18A10014A)
Donor diseases:
late-onset Alzheimer's disease
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi254-A
(SFC143-03-08)
Donor diseases:
Alzheimer disease
STBCi015-A
(SFC809-03-01)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi269-A
(BPC943-03-03)
Donor diseases:
Alzheimer disease
STBCi270-A
(BPC944-03-04)
Donor diseases:
Alzheimer disease
STBCi271-A
(BPC936-03-07)
Donor diseases:
Alzheimer disease
STBCi272-A
(BPC930-03-08)
Donor diseases:
Alzheimer disease
STBCi273-A
(BPC928-03-07)
Donor diseases:
Alzheimer disease
STBCi274-A
(BPC935-03-05)
Donor diseases:
Alzheimer disease
STBCi276-A
(BPC933-03-12)
Donor diseases:
Alzheimer disease
STBCi277-A
(BPC934-03-02)
Donor diseases:
Alzheimer disease
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line UKBi011-A. The set comprises the following APOE genotypes: • UKBi011-A-1 (APOE KO) • UKBi011-A-2 (APOE 2/2) • UKBi011-A-3 (APOE 3/3) • UKBi011-A-4 (APOE 3/4) • UKBi011-A (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi037-A and STBCi006-A.

Provider

Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
Derivation country Denmark

External Databases

hPSCreg UKBi011-A-2
BioSamples SAMEA104243175
Cellosaurus CVCL_VN45
Wikidata Q98133951

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasia, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
APOE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
APOE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104132641

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UKBi011-A.
Passage number reprogrammed 26

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR-1
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 27

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388C>T
NP_000032.1:p.Arg130Cys
Homozygous
UKBi011-A-2 ApoE2/E2 is an isogenic mutant of UKBi011-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with other base changes at rs7412 these define a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed
APOE (target)
Isogenic modification
19q13.3
NM_000041.3:c.526C>T
NP_000032.1:p.Arg176Cys
Homozygous
UKBi011-A-2 ApoE2/E2 is an isogenic mutant of UKBi011-A (ApoE4/E4); The genotype at base position described by rs7412 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with other base changes at rs429358 these define a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed