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STBCi006-A

SFC140-04-01

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name STBCi006-A
Alternative name(s)
SFC140-04-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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(SFC851-03-04)
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late-onset Alzheimer's disease
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(SFC801-03-01)
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(SFC802-03-06)
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APP
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STBCi011-A
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PSEN1
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STBCi011-B
(SFC803-03-02)
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STBCi014-B
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STBCi264-A
(SFC142-03-01)
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(BPC937-03-06)
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STBCi284-A
(BPC939-03-01)
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STBCi285-A
(BPC940-03-08)
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STBCi300-A
(BPC932-03-03)
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Alzheimer disease
STBCi301-A
(BPC949-04-11)
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Alzheimer disease
STBCi302-A
(BPC945-03-01)
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Alzheimer disease
STBCi032-A
(SFC042-03-01)
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STBCi032-B
(SFC042-03-03)
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STBCi314-A
(BPC335-03-08)
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STBCi045-A
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STBCi045-B
(SFC039-03-02)
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(SFC039-03-03)
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(LC56A10012A)
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(SFC057-07-02)
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STBCi097-A
(SFC055-04-02)
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Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi006-A
BioSamples SAMEA104133818
Cellosaurus CVCL_RB40
Wikidata Q54956332

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
APOE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
APOE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA104133819

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Source cell line lot number
SF140
Age of donor (at collection) 70-74
Collected in 2014

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unknown

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
day 4 post thaw

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX; no abnormalities detected
Passage number: 28
Karyotyping method: G-Banding

Other Genotyping (Cell Line)