STBCi025-A

SFC834-03-01

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name STBCi025-A
Alternative name(s)
SFC834-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi025-A
BioSamples SAMEA104493656
Cellosaurus CVCL_RB82
CLO CLO_0101755
Wikidata Q54956405

General Information

Publications View all related publications on hPSCreg (2)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson disease (primary disease)
Genetic variants
GBA1 (target)
GBA N370S

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493657

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
C15 LOH as observed in parent fibroblast; also modest allelic imbalance C6
Passage number: 21
Karyotyping method: Molecular karyotyping by SNP array
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Other Genotyping (Cell Line)