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STBCi025-A

SFC834-03-01

iPSC line

Not-for-profit fee: £1400 per vial
No longer available
The cell line was withdrawn.

General#

Cell Line

hPSCreg name STBCi025-A
Alternative name(s)
SFC834-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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Parkinson disease
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STBCi042-C
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STBCi088-B
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STBCi088-C
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GBA1
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Parkinson disease
UOXFi002-A
(MK082-26)
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(SFC871-03-04)
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UOXFi003-A
(MK088-1)
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GBA1
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Parkinson disease
UOXFi001-A
(MK071-1)
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GBA1
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Parkinson disease
UOXFi003-C
(MK088-7)
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GBA1
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UOXFi001-C
(MK071-5)
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(MK071-6)
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STBCi024-A
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(SFC031-03-03)
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ESi004-A
([GD]FiPS-4F-21c)
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GBA1
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EDi001-A-1
(AST22-C, AST23-C)
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Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
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SNCA, SNCA, SNCA, SNCA
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Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
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Parkinson disease
STBCi023-A
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STBCi023-C
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STBCi040-A
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Parkinson disease
STBCi040-B
(SFC029-03-08)
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STBCi041-A
(SFC081-03-02)
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STBCi043-A
(SFC120-03-03)
Donor diseases:
Parkinson disease
STBCi043-B
(SFC120-03-04)
Donor diseases:
Parkinson disease
STBCi040-C
(SFC029-03-01)
Donor diseases:
Parkinson disease
STBCi041-B
(SFC081-03-04)
Donor diseases:
Parkinson disease
STBCi043-C
(SFC120-03-02)
Donor diseases:
Parkinson disease
STBCi083-A
(SFC830-04-09)
Donor's gene variants:
SNCA
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Parkinson disease
STBCi086-A
(SFC842-03-02)
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Parkinson disease
STBCi086-B
(SFC842-03-07)
Donor diseases:
Parkinson disease

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi025-A
BioSamples SAMEA104493656
Cellosaurus CVCL_RB82
Wikidata Q54956405

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
GBA1 (target)
GBA N370S

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493657

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
C15 LOH as observed in parent fibroblast; also modest allelic imbalance C6
Passage number: 21
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)