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UOXFi001-D

MK071-6

iPSC line

The cell line has been recalled.

General#

Cell Line

hPSCreg Name UOXFi001-D
Alternative name(s)
MK071-6
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Oxford (UOXF)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg UOXFi001-D
BioSamples SAMEA4454476
Cellosaurus CVCL_EE30
CLO CLO_0101599
Wikidata Q54991435

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson disease
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
GBA (target)
1q22
NM_000157.3:c.1226A>G
NP_000148.2:p.Asn409Ser
Heterozygous
Heterozygous GBA N370S. N322S; ASN370SER

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4453898

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 70-74
Collected in 2011
Passage number reprogrammed 4

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
Pluripotency Score Novelty Score
22.39 1.44
Morphology pictures

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 13
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array

Other Genotyping (Cell Line)