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UOXFi002-B

MK082-30

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name UOXFi002-B
Alternative name(s)
MK082-30
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UOXFi002-A
(MK082-26)
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GBA1
Donor diseases:
Parkinson disease
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GBA1
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Parkinson disease
STBCi025-C
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STBCi085-A
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GBA1
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Parkinson disease
STBCi088-A
(SFC872-03-05)
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GBA1
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Parkinson disease
UOXFi003-C
(MK088-7)
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GBA1
Donor diseases:
Parkinson disease
STBCi084-A
(SFC871-03-12)
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GBA1
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Parkinson disease
STBCi084-C
(SFC871-03-09)
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GBA1
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Parkinson disease
UOXFi001-B
(MK071-3)
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GBA1
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Parkinson disease
STBCi025-B
(SFC834-03-03)
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GBA1
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Parkinson disease
STBCi042-A
(SFC848-03-02)
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GBA1
Donor diseases:
Parkinson disease
STBCi042-B
(SFC848-03-04)
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GBA1
Donor diseases:
Parkinson disease
STBCi042-C
(SFC848-03-06)
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GBA1
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Parkinson disease
STBCi085-B
(SFC866-03-02)
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GBA1
Donor diseases:
Parkinson disease
STBCi085-C
(SFC866-03-05)
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GBA1
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Parkinson disease
STBCi088-B
(SFC872-03-02)
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GBA1
Donor diseases:
Parkinson disease
STBCi088-C
(SFC872-03-03)
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GBA1
Donor diseases:
Parkinson disease
STBCi084-B
(SFC871-03-04)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-A
(MK071-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-C
(MK071-5)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-D
(MK071-6)
Donor's gene variants:
GBA1
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Parkinson disease
ESi004-A
([GD]FiPS-4F-21c)
Donor's gene variants:
GBA1
Donor diseases:
Gaucher disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease

Provider

Depositor University of Oxford (UOXF)
Distributors
EBiSC

External Databases

hPSCreg UOXFi002-B
BioSamples SAMEA4458549
Cellosaurus CVCL_IJ05
Wikidata Q54991437

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 50-54

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
GBA1 (target)
1q22
NM_000157.3:c.1226A>G
NP_000148.2:p.Asn409Ser
Heterozygous
Heterozygous GBA N370S. N322S; ASN370SER

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA4458548

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 50-54
Collected in 2011
Passage number reprogrammed 6

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-4
Yes
SSEA-1
No
Pluripotency Score Novelty Score
15.37 1.46
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 12
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array

Other Genotyping (Cell Line)