UOXFi003-A
MK088-1
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UOXFi003-A |
Alternative name(s) |
MK088-1
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
|
Depositor | University of Oxford (UOXF) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | UOXFi003-A |
BioSamples | SAMEA4454477 |
Cellosaurus | CVCL_EE31 |
ECACC | 66540391 |
CLO | CLO_0101600 |
Wikidata | Q54991439 |
General Information |
|
Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Age of donor (at collection) | 45-49 |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor | |
All cell lines of this donor's relatives |
Has brother:
|
External Databases (Donor) |
|
BioSamples | SAMEA4454478 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 45-49 |
Collected in | 2011 |
Passage number reprogrammed | 5 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Yes |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 20 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium | mTeSR™ 1 |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
|
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SSEA-4 |
Yes |
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SSEA-1 |
No |
|
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POU5F1 (OCT-4) |
Yes |
|
Pluripotency Score | Novelty Score | Link to microarray data |
23.43 | 1.67 | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425 |
Report
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 11
Karyotyping method:
Illumina Human CytoSNP-12v2.1 bead chip array
|
Other Genotyping (Cell Line) |