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UOXFi003-A

MK088-1

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name UOXFi003-A
Alternative name(s)
MK088-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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GBA1
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Parkinson disease
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UOXFi001-C
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STBCi042-B
(SFC848-03-04)
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GBA1
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Parkinson disease
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STBCi088-B
(SFC872-03-02)
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Parkinson disease
STBCi088-C
(SFC872-03-03)
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ESi004-A
([GD]FiPS-4F-21c)
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GBA1
Donor diseases:
Gaucher disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
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Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
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Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease

Provider

Depositor University of Oxford (UOXF)
Distributors
EBiSC

External Databases

hPSCreg UOXFi003-A
BioSamples SAMEA4454477
Cellosaurus CVCL_EE31
Wikidata Q54991439

General Information

Publications
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
GBA1 (target)
1q22
NM_000157.3:c.1226A>G
NP_000148.2:p.Asn409Ser
Heterozygous
Heterozygous GBA N370S. N322S; ASN370SER

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA4454478

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Collected in 2011
Passage number reprogrammed 5

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Pluripotency Score Novelty Score Link to microarray data
23.43 1.67 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53425
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 11
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array

Other Genotyping (Cell Line)