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UOXFi003-C

MK088-7

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
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General#

Cell Line

hPSCreg name UOXFi003-C
Alternative name(s)
MK088-7
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UOXFi003-A
(MK088-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi002-B
(MK082-30)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi002-A
(MK082-26)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-B
(MK071-3)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi025-C
(SFC834-03-10)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi085-A
(SFC866-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi088-A
(SFC872-03-05)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-A
(SFC871-03-12)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-C
(SFC871-03-09)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-A
(MK071-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-C
(MK071-5)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-D
(MK071-6)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi025-B
(SFC834-03-03)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi042-A
(SFC848-03-02)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi042-B
(SFC848-03-04)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi042-C
(SFC848-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi085-B
(SFC866-03-02)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi085-C
(SFC866-03-05)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi088-B
(SFC872-03-02)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi088-C
(SFC872-03-03)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-B
(SFC871-03-04)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
ESi004-A
([GD]FiPS-4F-21c)
Donor's gene variants:
GBA1
Donor diseases:
Gaucher disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease

Provider

Depositor University of Oxford (UOXF)
Distributors
EBiSC

External Databases

hPSCreg UOXFi003-C
BioSamples SAMEA4454479
Cellosaurus CVCL_EE32
Wikidata Q54991442

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
GBA1 (target)
1q22
NM_000157.3:c.1226A>G
NP_000148.2:p.Asn409Ser
Heterozygous
Heterozygous GBA N370S. N322S; ASN370SER

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA4454478

hIPSC Derivation#

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Collected in 2011
Passage number reprogrammed 4

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
Pluripotency Score Novelty Score
36.84 1.48
Morphology pictures

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 12
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array

Other Genotyping (Cell Line)