STBCi017-C

SFC811-03-05

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name STBCi017-C
Alternative name(s)
SFC811-03-05
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi017-A
(SFC811-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
RCi001-A
(Thp3C-6, RCi88)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi001-B
(RCi89, Thp3C-9)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
STBCi035-C
(SFC813-03-07)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi129-A
(SFC137-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
RCi002-A
(FP5C-14, RCi92)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-B
(FP5C-16, RCi93)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi003-A
(EM2-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi004-A
(EM2-7F)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi001-A
(EM1-3F)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi002-A
(EM1-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
STBCi018-A
(SFC815-03-06)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi018-B
(SFC815-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi018-C
(SFC815-03-02)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi059-A
(SFC015-01-05)
Donor diseases:
neuropathy
STBCi059-B
(SFC015-01-06)
Donor diseases:
neuropathy
STBCi059-C
(SFC015-01-10)
Donor diseases:
neuropathy
STBCi049-A
(SFC040-03-02)
Donor diseases:
neuropathy
STBCi049-B
(SFC040-03-03)
Donor diseases:
neuropathy
STBCi050-A
(SFC041-04-01)
Donor diseases:
neuropathy
STBCi050-B
(SFC041-04-02)
Donor diseases:
neuropathy
STBCi049-C
(SFC040-03-01)
Donor diseases:
neuropathy
STBCi050-C
(SFC041-04-03)
Donor diseases:
neuropathy
STBCi104-A
(SFC836-03-01)
Donor diseases:
neuropathy
STBCi037-B
(SFC816-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-C
(SFC816-03-13)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi017-C
BioSamples SAMEA104493632
ECACC 66540672
Cellosaurus CVCL_RB67
CLO CLO_0101740
Wikidata Q54956382

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
neuropathy (primary disease)
Genetic variants
SCN9A (target)
SCN9A heterozygotes for a) Premature stop codon in coding exon 15 p.R830X and b) A 1 bp deletion within coding exon 26 which induces a frameshift at position 1773 in the C terminal domain of the channel c.2488C>T

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493630

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 25-29

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)