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RCi001-B

RCi89, Thp3C-9

iPSC line

Re-stocking
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name RCi001-B
Alternative name(s)
RCi89, Thp3C-9
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider
Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg RCi001-B
BioSamples SAMEA3106040
Cellosaurus CVCL_9S42
ECACC 66540048
CLO CLO_0101554
Wikidata Q54949498

General Information
Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information
Sex male

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Primary erythromelalgia
Primary erythromelalgia in the OLS
The donor is affected.
Synonyms
  • Primary erythermalgia
Genetic variants
SCN9A (target)
2q24.3
Heterozygous
In the publication PMID:27099175, heterozygous point mutations were confirmed by Sanger sequencing: V400M: GTG(V) >>ATG(M)

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA3105813

hIPSC Derivation#

General
Source cell type
erythroblast
erythroblast in the OLS
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Source cell type (free text) Blood - PBMC erythroblasts
Passage number reprogrammed NA

Reprogramming method
Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch
Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XY[14]/47,XY,+12[5]
RCi89_P15_12Nov2014.pdf
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes