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RCi001-A

Thp3C-6, RCi88

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name RCi001-A
Alternative name(s)
Thp3C-6, RCi88
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi001-B
(RCi89, Thp3C-9)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-A
(FP5C-14, RCi92)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-B
(FP5C-16, RCi93)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
STBCi017-A
(SFC811-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
(SFC811-03-05)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-C
(SFC813-03-07)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi129-A
(SFC137-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy

Provider

Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC

External Databases

hPSCreg RCi001-A
BioSamples SAMEA3105842
Cellosaurus CVCL_9S41
Wikidata Q54949496

General Information

Publications View all related publications on hPSCreg (3)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Primary erythermalgia
Genetic variants
SCN9A (target)
2q24.3
Heterozygous
In the publication PMID:27099175, heterozygous point mutations were confirmed by Sanger sequencing: V400M: GTG(V) >>ATG(M)
Disease associated phenotypes
  • Familial Erythromelalgia

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105813

hIPSC Derivation#

General

Source cell type
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Source cell type (free text) PBMC erythroblasts

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes