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RCi002-A

FP5C-14, RCi92

iPSC line

In Stock
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name RCi002-A
Alternative name(s)
FP5C-14, RCi92
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider
Depositor Pfizer Limited - Pfizer (PFIZ)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg RCi002-A
BioSamples SAMEA3105892
Cellosaurus CVCL_9S43
ECACC 66540049
CLO CLO_0101560
Wikidata Q54949499

General Information
Publications View all related publications on hPSCreg (3)
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Primary erythromelalgia
Primary erythromelalgia in the OLS
The donor is affected.
Synonyms
  • Primary erythermalgia
Genetic variants
SCN9A (target)
2q24.3
NM_002977.3:c.4345T>G
NP_002968.1:p.Phe1449Val
Heterozygous
VCV000006352.2
rs80356478
In the publication PMID:27099175, heterozygous point mutations were confirmed by Sanger sequencing: F1449V: TTC(F) >> GTG(V). See Table 1.
Disease associated phenotypes
  • Familial Erythromelalgia

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA3105889

hIPSC Derivation#

General
Source cell type
erythroblast
erythroblast in the OLS
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Source cell type (free text) Blood PBMC erythroblasts
Passage number reprogrammed NA

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch
Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46XX
RCi92_well_P12_27Oct2014.pdf
Passage number: P12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes