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STBCi017-A

SFC811-03-01

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name STBCi017-A
Alternative name(s)
SFC811-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
(SFC811-03-05)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-C
(SFC813-03-07)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi129-A
(SFC137-03-01)
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SCN9A
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neuropathy
STBCi018-A
(SFC815-03-06)
Donor's gene variants:
GARS1
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neuropathy
RCi001-A
(Thp3C-6, RCi88)
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SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi001-B
(RCi89, Thp3C-9)
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SCN9A, SCN9A
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RCi002-A
(FP5C-14, RCi92)
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SCN9A, SCN9A
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Primary erythromelalgia
RCi002-B
(FP5C-16, RCi93)
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SCN9A, SCN9A
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Primary erythromelalgia
STBCi059-A
(SFC015-01-05)
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neuropathy
STBCi059-B
(SFC015-01-06)
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neuropathy
STBCi059-C
(SFC015-01-10)
Donor diseases:
neuropathy
STBCi060-A
(SFC038-07-05)
Donor diseases:
neuropathy
STBCi060-B
(SFC038-07-06)
Donor diseases:
neuropathy
STBCi060-C
(SFC038-07-07)
Donor diseases:
neuropathy
STBCi182-A
(SFC248-03-01)
Donor diseases:
neuropathy
STBCi184-A
(SFC251-03-01)
Donor diseases:
neuropathy
STBCi018-B
(SFC815-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi018-C
(SFC815-03-02)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi225-A
(SFC365-03-01)
Donor diseases:
neuropathy
STBCi232-A
(SFC375-03-01)
Donor diseases:
neuropathy
STBCi049-A
(SFC040-03-02)
Donor diseases:
neuropathy
STBCi049-B
(SFC040-03-03)
Donor diseases:
neuropathy
STBCi050-A
(SFC041-04-01)
Donor diseases:
neuropathy
STBCi050-B
(SFC041-04-02)
Donor diseases:
neuropathy
STBCi049-C
(SFC040-03-01)
Donor diseases:
neuropathy
STBCi050-C
(SFC041-04-03)
Donor diseases:
neuropathy
STBCi181-A
(SFC247-03-01)
Donor diseases:
neuropathy
STBCi183-A
(SFC250-03-01)
Donor diseases:
neuropathy
STBCi195-A
(SFC286-03-01)
Donor diseases:
neuropathy

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi017-A
BioSamples SAMEA104493629
Cellosaurus CVCL_RB65
Wikidata Q54956380

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Genetic variants
SCN9A (target)
SCN9A heterozygotes for a) Premature stop codon in coding exon 15 p.R830X and b) A 1 bp deletion within coding exon 26 which induces a frameshift at position 1773 in the C terminal domain of the channel c.2488C>T

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493630

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 25-29

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)