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STBCi018-C

SFC815-03-02

iPSC line

Re-stocking
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name STBCi018-C
Alternative name(s)
SFC815-03-02
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi018-A
(SFC815-03-06)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi018-B
(SFC815-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-B
(SFC816-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-C
(SFC816-03-13)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-A
(SFC816-03-02)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi017-A
(SFC811-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
(SFC811-03-05)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi059-A
(SFC015-01-05)
Donor diseases:
neuropathy
STBCi059-B
(SFC015-01-06)
Donor diseases:
neuropathy
STBCi059-C
(SFC015-01-10)
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi049-A
(SFC040-03-02)
Donor diseases:
neuropathy
STBCi049-B
(SFC040-03-03)
Donor diseases:
neuropathy
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi050-A
(SFC041-04-01)
Donor diseases:
neuropathy
STBCi050-B
(SFC041-04-02)
Donor diseases:
neuropathy
STBCi049-C
(SFC040-03-01)
Donor diseases:
neuropathy
STBCi050-C
(SFC041-04-03)
Donor diseases:
neuropathy
STBCi104-A
(SFC836-03-01)
Donor diseases:
neuropathy

Provider
Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg STBCi018-C
BioSamples SAMEA104493636
ECACC 66540675
Cellosaurus CVCL_RB70
CLO CLO_0101743
Wikidata Q54956386

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex male
Age of donor (at collection) 35-39

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
neuropathy (primary disease)
neuropathy in the OLS
Genetic variants
GARS1 (target)
7p14.3
GARS S581L

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA104493634

hIPSC Derivation#

General
Source cell type
fibroblast
fibroblast in the OLS
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 35-39

Reprogramming method
Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)