If the iPSCs you would like to access are currently listed as unavailable or
you would like information on local partners in USA, Australia, New Zealand or Japan who can support order and delivery,
please get in touch via
Contact@EBiSC.org.
WTSIi195-B
HPSI0414i-eevy_7
iPSC line
The cell line was withdrawn.
General#
iPSC Line |
|
| hPSCreg name | WTSIi195-B |
| Alternative name(s) |
HPSI0414i-eevy_7
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
|
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
|
| Derivation country | United Kingdom |
External iPSC Databases |
|
| hPSCreg | WTSIi195-B |
| BioSamples | SAMEA3848092 |
| HipSci | HPSI0414i-eevy_7 |
| Cellosaurus | CVCL_AJ88 |
| Wikidata | Q54890701 |
General iPSC Information |
|
| This EBiSC iPSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 15-19 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2464817 |
| HipSci | HPSI-eevy |
iPSC Derivation#
General |
|
| Source cell type |
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
|
| Source cell origin |
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
|
| Age of donor (at collection) | 15-19 |
| Collected in | 2015 |
| Source cell line vendor | University of Exeter Medical School |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
iPSC Characterisation#
Analysis of undifferentiated iPSCs
| Pluripotency Score | Novelty Score | |
| 28.76 | 1.509 |
Report
HPSI-eevy.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-eevy.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (iPSC Line) |
|
| Has the iPSC line karyotype been analysed? |
No
|
Other Genotyping (iPSC Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001001140
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-eevy_7 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149 Text file with probe intensities |