The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

WTSIi195-B

HPSI0414i-eevy_7

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi195-B
Alternative name(s)
HPSI0414i-eevy_7
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi195-A
(HPSI0414i-eevy_6)
Donor diseases:
monogenic diabetes
WTSIi204-A
(HPSI0414i-oulr_2)
Donor diseases:
monogenic diabetes
WTSIi196-A
(HPSI0514i-eoxi_6)
Donor diseases:
monogenic diabetes
WTSIi200-A
(HPSI0414i-kefk_2)
Donor diseases:
monogenic diabetes
WTSIi202-A
(HPSI0714i-oebj_1)
Donor diseases:
monogenic diabetes
WTSIi194-A
(HPSI0614i-dixh_2)
Donor diseases:
monogenic diabetes
WTSIi197-A
(HPSI0714i-fasu_2)
Donor diseases:
monogenic diabetes
WTSIi199-A
(HPSI0614i-guyj_2)
Donor diseases:
monogenic diabetes
WTSIi205-A
(HPSI0514i-pulk_1)
Donor diseases:
monogenic diabetes
WTSIi198-A
(HPSI0414i-fuai_1)
Donor diseases:
monogenic diabetes
WTSIi201-A
(HPSI0614i-koqx_1)
Donor diseases:
monogenic diabetes
WTSIi192-A
(HPSI0414i-biiw_1)
Donor diseases:
monogenic diabetes
WTSIi193-A
(HPSI0414i-cekz_1)
Donor diseases:
monogenic diabetes

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi195-B
BioSamples SAMEA3848092
HipSci HPSI0414i-eevy_7
Cellosaurus CVCL_AJ88
Wikidata Q54890701

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 15-19
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • monogenic diabetes mellitus
  • monogenic diabetes
  • rare genetic diabetes mellitus

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2464817
HipSci HPSI-eevy

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
  • peripheral blood mononuclear cell
Source cell origin
A fluid that is composed of blood plasma and erythrocytes.
Age of donor (at collection) 15-19
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
28.76 1.509

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-eevy_7
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls