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WTSIi195-B

HPSI0414i-eevy_7

iPSC line

Not-for-profit fee: £1400 per vial
No longer available
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi195-B
Alternative name(s)
HPSI0414i-eevy_7
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi195-A
(HPSI0414i-eevy_6)
Donor diseases:
monogenic diabetes
WTSIi204-A
(HPSI0414i-oulr_2)
Donor diseases:
monogenic diabetes
WTSIi196-A
(HPSI0514i-eoxi_6)
Donor diseases:
monogenic diabetes
WTSIi200-A
(HPSI0414i-kefk_2)
Donor diseases:
monogenic diabetes
WTSIi202-A
(HPSI0714i-oebj_1)
Donor diseases:
monogenic diabetes
WTSIi194-A
(HPSI0614i-dixh_2)
Donor diseases:
monogenic diabetes
WTSIi197-A
(HPSI0714i-fasu_2)
Donor diseases:
monogenic diabetes
WTSIi199-A
(HPSI0614i-guyj_2)
Donor diseases:
monogenic diabetes
WTSIi205-A
(HPSI0514i-pulk_1)
Donor diseases:
monogenic diabetes
WTSIi198-A
(HPSI0414i-fuai_1)
Donor diseases:
monogenic diabetes
WTSIi201-A
(HPSI0614i-koqx_1)
Donor diseases:
monogenic diabetes
WTSIi192-A
(HPSI0414i-biiw_1)
Donor diseases:
monogenic diabetes
WTSIi193-A
(HPSI0414i-cekz_1)
Donor diseases:
monogenic diabetes

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi195-B
BioSamples SAMEA3848092
HipSci HPSI0414i-eevy_7
Cellosaurus CVCL_AJ88
Wikidata Q54890701

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 15-19
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • monogenic diabetes mellitus
  • monogenic diabetes
  • rare genetic diabetes mellitus

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2464817
HipSci HPSI-eevy

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
  • peripheral blood mononuclear cell
Source cell origin
A fluid that is composed of blood plasma and erythrocytes.
Age of donor (at collection) 15-19
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
28.76 1.509

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-eevy_7
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls