WTSIi202-A

HPSI0714i-oebj_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi202-A
Alternative name(s)
HPSI0714i-oebj_1
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi202-A
BioSamples SAMEA3355551
HipSci HPSI0714i-oebj_1
ECACC 77650350, 66540437
Cellosaurus CVCL_AG80
CLO CLO_0102032
Wikidata Q54891322

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Ethnicity Middle East

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2663616
HipSci HPSI-oebj

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
15.076 1.41

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-oebj_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Imputed and phased genotypes