WTSIi197-A
HPSI0714i-fasu_2
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | WTSIi197-A |
Alternative name(s) |
HPSI0714i-fasu_2
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
|
Depositor | Wellcome Sanger Institute (WTSI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
Derivation country | United Kingdom |
External Databases |
|
hPSCreg | WTSIi197-A |
BioSamples | SAMEA3355537 |
HipSci | HPSI0714i-fasu_2 |
ECACC | 77650345, 66540432 |
Cellosaurus | CVCL_AG76 |
CLO | CLO_0102027 |
Wikidata | Q54891298 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 20-24 |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA2663620 |
HipSci | HPSI-fasu |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 20-24 |
Collected in | 2015 |
Source cell line vendor | University of Exeter Medical School |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Passage method | EDTA |
Surface coating | Vitronectin |
O2 concentration | 20 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-1 |
No |
|
||||
SSEA-4 |
Yes |
|
||||
TRA 1-60 |
Yes |
|
Pluripotency Score | Novelty Score | |
33.78 | 1.343 |
Report
HPSI-fasu.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-fasu.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001001140
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-fasu_2 Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149 Text file with probe intensities |