WTSIi205-A

HPSI0514i-pulk_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi205-A
Alternative name(s)
HPSI0514i-pulk_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi194-A
(HPSI0614i-dixh_2)
Donor diseases:
monogenic diabetes
WTSIi197-A
(HPSI0714i-fasu_2)
Donor diseases:
monogenic diabetes
WTSIi199-A
(HPSI0614i-guyj_2)
Donor diseases:
monogenic diabetes
WTSIi196-A
(HPSI0514i-eoxi_6)
Donor diseases:
monogenic diabetes
WTSIi198-A
(HPSI0414i-fuai_1)
Donor diseases:
monogenic diabetes
WTSIi200-A
(HPSI0414i-kefk_2)
Donor diseases:
monogenic diabetes
WTSIi201-A
(HPSI0614i-koqx_1)
Donor diseases:
monogenic diabetes
WTSIi202-A
(HPSI0714i-oebj_1)
Donor diseases:
monogenic diabetes
WTSIi410-B
(HPSI0614i-zoio_1)
Donor diseases:
monogenic diabetes
WTSIi417-B
(HPSI0614i-voxu_2)
Donor diseases:
monogenic diabetes
WTSIi444-A
(HPSI0714i-riya_2)
Donor diseases:
monogenic diabetes
WTSIi396-A
(HPSI0414i-kodf_2)
Donor diseases:
monogenic diabetes
WTSIi398-A
(HPSI0414i-rauj_1)
Donor diseases:
monogenic diabetes
WTSIi400-A
(HPSI0414i-tout_1)
Donor diseases:
monogenic diabetes
WTSIi401-A
(HPSI0714i-meet_3)
Donor diseases:
monogenic diabetes
WTSIi408-A
(HPSI0414i-niim_3)
Donor diseases:
monogenic diabetes
WTSIi408-B
(HPSI0414i-niim_5)
Donor diseases:
monogenic diabetes
WTSIi410-A
(HPSI0614i-zoio_2)
Donor diseases:
monogenic diabetes
WTSIi203-A
(HPSI0714i-oojs_1)
Donor diseases:
monogenic diabetes
WTSIi477-A
(HPSI0514i-kooz_5)
Donor diseases:
monogenic diabetes
WTSIi417-A
(HPSI0614i-voxu_1)
Donor diseases:
monogenic diabetes
WTSIi430-B
(HPSI0514i-aecv_3)
Donor diseases:
monogenic diabetes
WTSIi428-A
(HPSI0714i-lutt_6)
Donor diseases:
monogenic diabetes
WTSIi430-A
(HPSI0514i-aecv_2)
Donor diseases:
monogenic diabetes
WTSIi192-A
(HPSI0414i-biiw_1)
Donor diseases:
monogenic diabetes
WTSIi193-A
(HPSI0414i-cekz_1)
Donor diseases:
monogenic diabetes

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi205-A
BioSamples SAMEA3282740
HipSci HPSI0514i-pulk_1
ECACC 77650349, 66540440
Cellosaurus CVCL_AF82
CLO CLO_0102034
Wikidata Q54890917

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2548025
HipSci HPSI-pulk

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 50-54
Collected in 2014
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
21.548 1.394

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-pulk_1
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts