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WTSIi194-A

HPSI0614i-dixh_2

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi194-A
Alternative name(s)
HPSI0614i-dixh_2
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi194-A
BioSamples SAMEA3283000
HipSci HPSI0614i-dixh_2
Cellosaurus CVCL_AG39
Wikidata Q54891115

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 75-79
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • monogenic diabetes mellitus
  • monogenic diabetes
  • rare genetic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2595433
HipSci HPSI-dixh

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 75-79
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
37.506 1.338

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0614i-dixh_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts