STBCi026-A-3
SFC840-03-03 LRRK2 WT/R1441C H3
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | STBCi026-A-3 |
Alternative name(s) |
SFC840-03-03 LRRK2 WT/R1441C H3
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
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Depositor | StemBANCC (STBC) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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hPSCreg | STBCi026-A-3 |
BioSamples | SAMEA5859481 |
ECACC | 66541207 |
Cellosaurus | CVCL_A9A0 |
CLO | CLO_0103008 |
Wikidata | Q102114947 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104493661 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
STBCi026-A.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR1 |
Passage method | EDTA |
Surface coating | Matrigel |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 °C |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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TRA 1-60 |
Yes |
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Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
No gross karyotypic abnormalities observed, 6 small indels as in parent line
Passage number: 31
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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