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STBCi026-A-3

SFC840-03-03 LRRK2 WT/R1441C H3

Gene-edited iPSC line

Not-for-profit fee: £1700 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: confluency
Magnification: 4x
Timepoint: confluency
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name STBCi026-A-3
Alternative name(s)
SFC840-03-03 LRRK2 WT/R1441C H3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi026-A-1
(SFC840-03-03 LRRK2-/-D10)
STBCi026-A-2
(SFC840-03-03 LRRK2-/-C11)
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi026-A
(SFC840-03-03)
STBCi026-B
(SFC840-03-01)
STBCi026-A-4
(SFC840-03-03-PITX3-GFP)
STBCi026-C
(SFC840-03-05)
STBCi026-D
(SFC840-03-06)
BIONi037-A-2
(BIONi037-A ApoE2/2 #M10-7)
BIONi037-A-3
(BIONi037-A ApoE3/4 #P10-22)
BIONi037-A-4
(BIONi037-A ApoE4/4 #I10-53)
BIONi037-A-1
(16423 ApoE KO)

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi026-A-3
BioSamples SAMEA5859481
Cellosaurus CVCL_A9A0
Wikidata Q102114947

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

hIPSC Derivation#

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR1
Passage method EDTA
Surface coating Matrigel
O2 concentration 21
CO2 concentration 5
Temperature 37 °C
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No gross karyotypic abnormalities observed, 6 small indels as in parent line
Passage number: 31
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic modifications
LRRK2 (target)
Variant
12q12
NM_198578.4:c.4321C>T
NP_940980.4:p.Arg1441Cys
Heterozygous
Introduction of R1441C variant into wildtype iPSC line generated from individual with no known disease. CRISPR/Cas9-mediated double-strand break generated close locus (R1441C mutation) and homology-directed repair with donor sequence. See cell line CoA under 'Characterisation'.