STBCi026-A-1
SFC840-03-03 LRRK2-/-D10
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | STBCi026-A-1 |
Alternative name(s) |
SFC840-03-03 LRRK2-/-D10
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
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Depositor | StemBANCC (STBC) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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hPSCreg | STBCi026-A-1 |
BioSamples | SAMEA5859479 |
ECACC | 66541205 |
CLO | CLO_0102948 |
Cellosaurus | CVCL_A8Z8 |
Wikidata | Q102114944 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104493661 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
STBCi026-A.
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR1 |
Passage method | EDTA |
Surface coating | Matrigel |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 °C |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
Medium |
mTeSR™ 1
|
Characterisation#
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
Karyotype abnormalities: none compared to fibroblasts
Passage number: 31
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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