If the cells you would like to access are currently listed as unavailable
or you are ordering from outside of Europe please get in touch via
Contact@EBiSC.org.
STBCi026-A-1
SFC840-03-03 LRRK2-/-D10
Gene-edited iPSC line
Timepoint: confluency
Magnification: 4x
Magnification: 4x
Timepoint: confluency
Magnification: 10x
Magnification: 10x
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
The EBiSC Access and Use Agreement must be completed along with an individual
Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org
for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg name | STBCi026-A-1 |
| Alternative name(s) |
SFC840-03-03 LRRK2-/-D10
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
STBCi026-A-3 (SFC840-03-03 LRRK2 WT/R1441C H3) STBCi026-A-2 (SFC840-03-03 LRRK2-/-C11) STBCi004-B-1 (SFC832-03-06 LRRK2WT/WT C47) Donor's gene variants: LRRK2 Donor diseases: Parkinson disease STBCi026-A (SFC840-03-03) STBCi026-B (SFC840-03-01) STBCi026-A-4 (SFC840-03-03-PITX3-GFP) STBCi026-C (SFC840-03-05) STBCi026-D (SFC840-03-06) BIONi037-A-2 (BIONi037-A ApoE2/2 #M10-7) BIONi037-A-3 (BIONi037-A ApoE3/4 #P10-22) BIONi037-A-4 (BIONi037-A ApoE4/4 #I10-53) BIONi037-A-1 (16423 ApoE KO) |
Provider |
|
| Depositor | StemBANCC (STBC) |
| Distributors |
EBiSC
|
External Databases |
|
| hPSCreg | STBCi026-A-1 |
| BioSamples | SAMEA5859479 |
| Cellosaurus | CVCL_A8Z8 |
| Wikidata | Q102114944 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA104493661 |
hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
STBCi026-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | mTeSR1 |
| Passage method | EDTA |
| Surface coating | Matrigel |
| O2 concentration | 21 |
| CO2 concentration | 5 |
| Temperature | 37 °C |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| Medium |
mTeSR™ 1
|
Characterisation#
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Karyotype abnormalities: none compared to fibroblasts
Passage number: 31
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|