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STBCi026-A-4

SFC840-03-03-PITX3-GFP

Gene-edited iPSC line

Not-for-profit fee: £1700 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name STBCi026-A-4
Alternative name(s)
SFC840-03-03-PITX3-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi026-A
(SFC840-03-03)
STBCi026-A-1
(SFC840-03-03 LRRK2-/-D10)
STBCi026-A-3
(SFC840-03-03 LRRK2 WT/R1441C H3)
STBCi026-B
(SFC840-03-01)
STBCi026-A-2
(SFC840-03-03 LRRK2-/-C11)
STBCi026-C
(SFC840-03-05)
STBCi026-D
(SFC840-03-06)

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi026-A-4
BioSamples SAMEA5859482
Cellosaurus CVCL_A4YP
Wikidata Q107116956

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493661

hIPSC Derivation#

General

The source cell information can be found in the parental cell line STBCi026-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation#

Differentiation Potency
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
TUJ-1
Yes
PITX3
Yes
Morphology
Dopaminergic neuronal differentiation

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
PITX3 (target)
Gene knock-in
10q24.32
GFP
10q24.32
PITX3-GFP tag inserted, single copy.
CRISPR-associated (CRISPR/Cas) System