STBCi026-A-2
SFC840-03-03 LRRK2-/-C11
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | STBCi026-A-2 |
| Alternative name(s) |
SFC840-03-03 LRRK2-/-C11
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | StemBANCC (STBC) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
| hPSCreg | STBCi026-A-2 |
| BioSamples | SAMEA5859480 |
| ECACC | 66541206 |
| CLO | CLO_0102949 |
| Cellosaurus | CVCL_A8Z9 |
| Wikidata | Q102114946 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA104493661 |
hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
STBCi026-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| Medium |
mTeSR™ 1
|
Characterisation#
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Karyotype abnormalities compared to original iPSC line: small amplification in C14q and deletion in C19q
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|