The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

WTSIi148-A

HPSI0114i-veui_3

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi148-A
Alternative name(s)
HPSI0114i-veui_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi148-A
BioSamples SAMEA2464772
HipSci HPSI0114i-veui_3
Cellosaurus CVCL_AE37
Wikidata Q54890453

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2397997
HipSci HPSI-veui

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
35.954 1.283

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-veui_3
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Genotyping array calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts