If the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe please get in touch via Contact@EBiSC.org.

WTSIi148-A

HPSI0114i-veui_3

iPSC line

Not-for-profit fee: £1400 per vial
No longer available
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi148-A
Alternative name(s)
HPSI0114i-veui_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi154-A
(HPSI0414i-lavd_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi159-A
(HPSI0714i-gibe_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi161-A
(HPSI0714i-rajk_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi167-A
(HPSI1013i-funy_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi033-A
(HPSI1013i-aetc_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi153-A
(HPSI0414i-puvg_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi156-A
(HPSI0414i-xugr_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi157-A
(HPSI0614i-eavo_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi162-A
(HPSI0914i-lopq_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi166-A
(HPSI0414i-wize_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi147-A
(HPSI1013i-mijn_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi151-A
(HPSI0414i-fixm_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi152-A
(HPSI0414i-eafb_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi158-A
(HPSI0914i-fuhz_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi165-A
(HPSI0414i-reng_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi034-A
(HPSI0913i-kojv_2)
Donor diseases:
Bardet-Biedl syndrome
WTSIi035-A
(HPSI0913i-gooj_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi149-A
(HPSI0913i-laia_4)
Donor diseases:
Bardet-Biedl syndrome
WTSIi150-A
(HPSI0414i-jetz_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi155-A
(HPSI0414i-hutq_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi160-A
(HPSI0914i-verf_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi163-A
(HPSI1014i-suok_3)
Donor diseases:
Bardet-Biedl syndrome

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi148-A
BioSamples SAMEA2464772
HipSci HPSI0114i-veui_3
Cellosaurus CVCL_AE37
Wikidata Q54890453

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2397997
HipSci HPSI-veui

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2014
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
35.954 1.283

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-veui_3
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Genotyping array calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts