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WTSIi147-A

HPSI1013i-mijn_2

iPSC line

Not-for-profit fee: £1400 per vial
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General#

Cell Line

hPSCreg name WTSIi147-A
Alternative name(s)
HPSI1013i-mijn_2
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi147-A
BioSamples SAMEA2398395
HipSci HPSI1013i-mijn_2
Cellosaurus CVCL_AH71
Wikidata Q54891562

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 35-39
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398140
HipSci HPSI-mijn

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 35-39
Collected in 2013
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
36.194 1.315

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1013i-mijn_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 6
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Genotyping array calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Imputed and phased genotypes
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Imputed and phased genotypes
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Text file with probe intensities