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WTSIi155-A

HPSI0414i-hutq_1

iPSC line

Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi155-A
Alternative name(s)
HPSI0414i-hutq_1
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi155-A
BioSamples SAMEA2777391
HipSci HPSI0414i-hutq_1
Cellosaurus CVCL_AF17
Wikidata Q54890708

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 25-29
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2482376
HipSci HPSI-hutq

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 25-29
Collected in 2014
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
32.68 1.434

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-hutq_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts