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WTSIi036-A

HPSI1113i-waus_1

iPSC line

No longer available
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi036-A
Alternative name(s)
HPSI1113i-waus_1
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi036-A
BioSamples SAMEA2469767
HipSci HPSI1113i-waus_1
Cellosaurus CVCL_AI05
Wikidata Q54891665

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2399191
HipSci HPSI-waus

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 40-44
Collected in 2014
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
22.462 1.213

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-waus_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Genotyping array calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Abundances of transcripts
RNA-seq
https://ega-archive.org/datasets/EGAD00001003531
Splice-aware STAR alignment
Genotyping array
https://ega-archive.org/datasets/EGAD00010000777
Imputed and phased genotypes
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003515
BWA alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145
Text file with probe intensities