UKKi025-C
NP0135-7
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | UKKi025-C |
Alternative name(s) |
NP0135-7
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UKKi035-B (NP0139-B, NP0139-6C) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Hypertrophic cardiomyopathy SCVIi074-A (SCVIi599C1) Donor's gene variants: BAG cochaperone 3 Donor diseases: Hypertrophic Cardiomyopathy SCVIi073-A (SCVIi574C2) Donor's gene variants: titin, BAG cochaperone 3, BAG cochaperone 3 Donor diseases: Dilated Cardiomyopathy FAMRCi006-A (LMNA T3) Donor's gene variants: LMNA Donor diseases: Emery-Dreifuss Muscular Dystrophy Dilated Cardiomyopathy FAMRCi006-B (LMNA T4) Donor's gene variants: LMNA Donor diseases: Emery-Dreifuss Muscular Dystrophy Dilated Cardiomyopathy |
Provider |
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Depositor | Klinikum der Universität zu Köln (UKK) |
Owner | Institute for Neurophysiology, Medical Faculty |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Germany |
External Databases |
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hPSCreg | UKKi025-C |
BioSamples | SAMEA17638918 |
Cellosaurus | CVCL_LD44 |
ECACC | 66540493 |
CLO | CLO_0101635 |
Wikidata | Q54990493 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 40-44 |
Ethnicity | caucasian / european |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Family history | the mother is also affected |
Is clinical information available? | Mutation: MYH7(Exon19); c.2156G>A; p.Arg719Gly |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
No larger chromosomal aberrations observed
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA17637418 |
hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 40-44 |
Collected in | 2016 |
Passage number reprogrammed | 0 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Notes on reprogramming vector detection | sendai virus absent |
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
|
Selection criteria for clones | morphology |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin | |||||||||
Feeder cells |
No |
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Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Essential 8™
Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
No larger chromosomal aberrations observed.
Passage number: 31
Karyotyping method:
Molecular karyotyping by SNP array
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
|