UKKi017-A

NP0075-8D

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi017-A
Alternative name(s)
NP0075-8D
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi017-B
(NP0075-10K)
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Hypertrophic cardiomyopathy
UKKi017-C
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Hypertrophic cardiomyopathy
UKKi025-A
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MYH7
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UKKi025-B
(NP0135-2)
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MYH7
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UKKi025-C
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UKKi018-C
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KCNH2
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Familial long QT syndrome
UKKi018-A
(NP0080-2B)
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KCNH2
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Familial long QT syndrome
UKKi019-A
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Familial long QT syndrome
UKKi018-B
(NP0080-6A)
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KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-B
(NP0081-11)
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KCNH2
Donor diseases:
Familial long QT syndrome
UKKi019-C
(NP0081-12C)
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KCNH2
Donor diseases:
Familial long QT syndrome
UKKi035-B
(NP0139-B, NP0139-6C)
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MYBPC3, MYBPC3
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Hypertrophic cardiomyopathy
UKKi016-A
(NP0078-10)
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UKKi023-A
(NP0126-1)
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Familial long QT syndrome
UKKi023-B
(NP0126-5)
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KCNH2
Donor diseases:
Familial long QT syndrome
UKKi023-C
(NP0126-6)
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KCNH2
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Familial long QT syndrome
ICANi001-A-1
(CDGEN1.16.40.5)
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hypertrophic cardiomyopathy
UKEi070-A-1
(Rep 28, Iso Co)
Donor diseases:
hypertrophic cardiomyopathy
UKEi070-A-2
(Bi-allelic mutant, Mut 103)
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hypertrophic cardiomyopathy
SCVIi074-A
(SCVIi599C1)
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BAG cochaperone 3
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Hypertrophic Cardiomyopathy
ICGi029-A
(HCM14fm6.2)
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Hypertrophic Cardiomyopathy
SCVIi073-A
(SCVIi574C2)
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titin, BAG cochaperone 3, BAG cochaperone 3
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Long QT Syndrome
PUMCHi002-A
(20180908R-AE3, PUMCH-IRC)
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Restrictive Cardiomyopathy
ITXi004-A
(MS381)
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Long QT Syndrome
FAMRCi006-A
(LMNA T3)
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LMNA
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Emery-Dreifuss Muscular Dystrophy
Dilated Cardiomyopathy
FAMRCi006-B
(LMNA T4)
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LMNA
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USFi003-A
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Dilated Cardiomyopathy
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Restrictive Cardiomyopathy
SCVIi037-A
(SCVI672)
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hypertrophic cardiomyopathy
UKKi031-A
(NP0138-8B)
Donor's gene variants:
TNNT2
Donor diseases:
Hypertrophic cardiomyopathy
UKKi031-B
(NP0138-19E)
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TNNT2
Donor diseases:
Hypertrophic cardiomyopathy
UKKi031-C
(NP0138-31B)
Donor's gene variants:
TNNT2
Donor diseases:
Hypertrophic cardiomyopathy
ICGi028-A
(HCM4fm5.2)
Donor diseases:
Hypertrophic Cardiomyopathy
ITXi003-A
(MS368)
Donor diseases:
Long QT Syndrome
UKKi029-A
(NP0077-6D)
Donor's gene variants:
KCNQ1
Donor diseases:
Prolonged QT interval
UKKi029-B
(NP0077-16E)
Donor's gene variants:
KCNQ1
Donor diseases:
Prolonged QT interval

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
Centre for Physiology and Pathophysiology, Institute for Neurophysiology
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Scottish Centre for Regenerative Medicine
Derivation country Germany

External Databases

hPSCreg UKKi017-A
BioSamples SAMEA17619418
Cellosaurus CVCL_LD22
ECACC 66540475
CLO CLO_0101627
Wikidata Q54990435

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
Genetic testing of the patient was not performed
The donor is affected.
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA17620168

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 50-54
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
Essential 8 supplement 50x 10 ml
Essential 8 Flex supplement 50x 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT 4
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
CD90
Yes
Morphology pictures
morphology pictures of iPS cell line NP0075-8D before banking and of the thawing control
morphology pictures of iPS cell line NP0075-8D before banking and of the thawing control
morphology pictures of iPS cell line NP0075-8D before banking and of the thawing control
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
expression of SOX17 in differentiated iPS cell line NP0075-8D
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0075-8D
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology
expression of NESTIN in differentiated iPS cell line NP0075-8D

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 36
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array