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UKKi017-C

NP0075-11B

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name UKKi017-C
Alternative name(s)
NP0075-11B
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKKi017-B
(NP0075-10K)
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi031-A
(NP0138-8B)
Donor's gene variants:
TNNT2
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi025-A
(NP0135-1)
Donor's gene variants:
MYH7
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi025-B
(NP0135-2)
Donor's gene variants:
MYH7
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi025-C
(NP0135-7)
Donor's gene variants:
MYH7
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi035-B
(NP0139-B, NP0139-6C)
Donor's gene variants:
MYBPC3, MYBPC3
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi031-B
(NP0138-19E)
Donor's gene variants:
TNNT2
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi031-C
(NP0138-31B)
Donor's gene variants:
TNNT2
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi035-A
(NP0139-A, NP0139-3E)
Donor's gene variants:
MYBPC3, MYBPC3
Donor diseases:
Rare hypertrophic cardiomyopathy
UKKi035-C
(NP0139-C, NP0139-24D)
Donor's gene variants:
MYBPC3, MYBPC3
Donor diseases:
Rare hypertrophic cardiomyopathy

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
Derivation country Germany

External Databases

hPSCreg UKKi017-C
BioSamples SAMEA17621668
Cellosaurus CVCL_LD24
Wikidata Q54990437

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Genetic testing of the patient was not performed
The donor is affected.
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA17620168

hIPSC Derivation#

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 50-54
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
Essential 8 supplement 50x 10 ml
Essential 8 Flex supplement 50x 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT 4
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
CD90
Yes
Morphology pictures
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
expression of SOX17 in differentiated iPS cell line NP0075-11B
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0075-11B
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Yes
Morphology
expression of NESTIN in differentiated iPS cell line NP0075-11B

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations observed
Passage number: 34
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768